Fig. 9. NF-κB acts upstream COX-2 and downstream PKC & ERK. Effect of (A) FTY720-P (7.5nM. 2hours) on the protein expression of IκB in cells pre-treated for 30 min with either a PKC inhibitor (calphostin C; 50nM) or an ERK inhibitor (PD98059; 50 µM). (B) Effect FTY720-P (7.5nM. 2hours) on the protein expression of COX-2 enzyme in cells pre-treated for 30 min with an inhibitor of the NF-κB (15 µM). Values are normalized to GAPDH using Image lab software. The blot is representative of an experiment repeated 3 times. Values are means ± SEM of 3 observations. Bars not sharing a common letter are considered significantly different from each other at p< 0.01.